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Image Search Results
Journal: Scientific Reports
Article Title: A lncRNA landscape in breast cancer reveals a potential role for AC009283.1 in proliferation and apoptosis in HER2-enriched subtype
doi: 10.1038/s41598-020-69905-z
Figure Lengend Snippet: ( A ) Real-time qPCR illustrating gene silencing by shRNA against AC009283.1 in SKBR3, a HER2-enriched cell line model. ( B ) Supervised hierarchical cluster analysis shows 158 differentially expressed genes after AC009283.1 knockdown (Foldchange < − 1.2, > 1.2 p-value < 0.05); we observed 94 up-regulated and 64 down-regulated genes in shRNA-2 vs NC and ( C ). Their distribution across the genome. ( D ) Ingenuity Pathway Analysis of 158 genes differentially expressed after knockdown of AC009283.1 in SKBR3 cells. ( E ) Enrichment pathway analysis of genes differently expressed in samples of HER2-enriched tumors from TCGA with high vs low expression of AC009283.1.
Article Snippet: Human BRCA cell lines included in the analyses (MCF7, ZR-75-1, MDA-MB-361,
Techniques: shRNA, Knockdown, Expressing
Journal: Scientific Reports
Article Title: A lncRNA landscape in breast cancer reveals a potential role for AC009283.1 in proliferation and apoptosis in HER2-enriched subtype
doi: 10.1038/s41598-020-69905-z
Figure Lengend Snippet: ( A ) Proliferation assay performed following knockdown of AC009283.1 with shRNA2, shRNA NC as negative control, and wildtype cell line. Cells were counted every day for four days. *p < 0.05, **p < 0.005, ***p < 0.0005, comparing with shRNA NC for each condition via Student’s t test. ( B ) Proliferation index using CFSE assay in SKBR3 cells with shRNA2 AC009283.1 and shRNA NC. *p < 0.05, **p < 0.005, ***p < 0.0005, comparing with shRNA NC for each condition via Student’s t test. ( C ) Representative CFSE flow-cytometry histograms showing the effect on in vitro SKBR-3 cell proliferation with shRNA NC and shRNA2 (AC009283.1 knock-down). ( D ) Cell cycle assay with flow-cytometry using (propidium iodide) PI on in vitro SKBR-3 cells, AC009283.1 knockdown causes accumulation of cells at S phase. *p < 0.05, **p < 0.005, ***p < 0.0005, comparing to shRNA NC for each condition via Student’s t test.
Article Snippet: Human BRCA cell lines included in the analyses (MCF7, ZR-75-1, MDA-MB-361,
Techniques: Proliferation Assay, Knockdown, shRNA, Negative Control, CFSE Assay, Flow Cytometry, In Vitro, Cell Cycle Assay